abstract：:The ciliated protozoan Paramecium harbours a particularly large diversity of microtubule networks, ranging from the elaborate and stable ciliary axonemes and basal bodies to very dynamic cytoplasmic, cortical or intranuclear arrays. Their organization and individual cycle of assembly/disassembly are well known and ext...

journal_title：Biology of the cell

authors： Dupuis-Williams P,Klotz C,Mazarguil H,Beisson J

更新日期：1996-01-01 00:00:00

abstract：:Regulated exocytosis of defensive secretory organelles, the trichocysts, as well as a transient Ca(2+)-influx can be induced in Paramecium by aminoethyldextran (Kerboeuf and Cohen, J Cell Biol (1990) 111, 2527). Knoll et al (Febs Lett (1992) 304, 265) reported that veratridine was also a secretagogue for Paramecium. H...

journal_title：Biology of the cell

authors： Kerboeuf D,Cohen J

更新日期：1996-01-01 00:00:00

abstract：:Extensive developmentally programmed DNA rearrangements, including thousands of internal deletions, occur in the differentiating somatic macronucleus in Tetrahymena thermophila. Some deletion systems involve the use of multiple alternative deletion sites. We report here the cloning and the sequences of three new alter...

journal_title：Biology of the cell

authors： Chau MF,Orias E

更新日期：1996-01-01 00:00:00

abstract：:One of the initial events required for the expression of cartilage-specific macromolecules in monolayer cultures is the reversion to the initial round shape of chondrocytes. Thus, considerable research efforts have focused on developing reliable procedures to maintain a round morphology of cultured chondrocytes. Our s...

journal_title：Biology of the cell

authors： Loty S,Forest N,Boulekbache H,Sautier JM

更新日期：1995-01-01 00:00:00

abstract：:The dynamics of change in mitochondria-rich (MR) cells density in the skin epithelium of Bufo viridis was studied on skin biopsies taken in vivo, throughout experimental periods lasting up to 3 months. When the bathing solution contained Cl-, MR cells' density (Dmrc) greatly decreased. There was one exception, when th...

journal_title：Biology of the cell

authors： Katz U,Gabby S

更新日期：1995-01-01 00:00:00

abstract：:In the present study we report the occurrence of chaperonins, cpn10 and cpn60, in Chromatium vinosum and rat hepatocytes, using specific polyclonal antibodies in conjunction with the protein A-gold immunocytochemical technique. As demonstrated by quantitative evaluations, the immunolabeling for cpn10 and cpn60 in C vi...

journal_title：Biology of the cell

authors： Vélez-Granell CS,Arias AE,Torres-Ruíz JA,Bendayan M

更新日期：1995-01-01 00:00:00

abstract：:Multidrug-resistant (MDR) variants of a human osteosarcoma cell line (U-2 OS) have been recently obtained by continuous exposure to doxorubicin (DX). The growth and phenotypic characteristics of these cell lines have been demonstrated to be related to the level of expression of P-glycoprotein. In this work, the morpho...

journal_title：Biology of the cell

authors： Zini N,Scotlandi K,Baldini N,Nini G,Sabatelli P,Maraldi N

更新日期：1995-01-01 00:00:00

abstract：:In order to determine whether the human lactotransferrin receptor recently described on platelets was also present on hematopoietic precursors, we investigated its presence and characteristics on the megakaryocytic Dami cell line. The reversible binding of human 5-(([2-(carbo(hydrazino)methyl]thio)acetyl)aminofluoresc...

journal_title：Biology of the cell

authors： Nillesse N,Pierce A,Lecocq M,Benaissa M,Spik G

更新日期：1994-01-01 00:00:00

abstract：:The isolation, characterization, and expression of a novel cDNA encoding a Trypanosoma cruzi polypeptide (TcAc2), homologous to various small stress proteins and glutathione S-transferases, are described. The deduced amino-acid sequence revealed two domains sharing 27% identity and an additional 27% similarity to each...

journal_title：Biology of the cell

authors： Schöneck R,Plumas-Marty B,Taibi A,Billaut-Mulot O,Loyens M,Gras-Masse H,Capron A,Ouaissi A

更新日期：1994-01-01 00:00:00

abstract：:Human pancreatic ductal cells of the Capan 1 cell line differentiate progressively during growth. After the exponential growth phase, the cells elongate and become polarized with their apical poles covered by microvilli and separated from the basolateral pole by tight junctions. In this stationary phase, they form dom...

journal_title：Biology of the cell

authors： Mahieu I,Becq F,Wolfensberger T,Gola M,Carter N,Hollande E

更新日期：1994-01-01 00:00:00

abstract：:Although there are many macromolecular complexes which play extremely important roles in biology, and despite continued progress in X-ray crystallographic and NMR methods, it is still very difficult to obtain atomic level structural information about such large assemblies. It is now clear that a powerful approach is t...

journal_title：Biology of the cell

authors： Wade RH,Hewat EA

更新日期：1994-01-01 00:00:00

abstract：:Lentil seedlings were grown for 28 h in space, on board Spacelab (IML 1 Mission) and growth of the primary root was analysed. The length of cortical cells was less in near weightlessness than on the 1 g centrifuge (flight control) and mitotic index was lower but there was no apparent perturbation in the mitosis. To fu...

journal_title：Biology of the cell

authors： Driss-Ecole D,Schoevaert D,Noin M,Perbal G

更新日期：1994-01-01 00:00:00

abstract：:The clonal rat rhabdomyosarcoma cell line BA-HAN-1C is composed of proliferating mononuclear cells, some of which spontaneously fuse to terminally differentiated myotube-like giant cells. This cell line has been shown to be susceptible to differentiation induction with all-trans retinoic acid (RA). Since it is still u...

journal_title：Biology of the cell

authors： Ramp U,Gerharz CD,Eifler E,Biesalski HK,Gabbert HE

更新日期：1994-01-01 00:00:00

abstract：:The mammalian spermatozoa are endowed with a unique cytoskeleton which consists both of ubiquitous and specific proteins, some of them arising from gene haploid transcription. In the head, a dense perinuclear layer is made of basic proteins (calicin, cylicin, etc) associated with calmodulin and actin remnants. In the ...

journal_title：Biology of the cell

authors： Fouquet JP,Kann ML

更新日期：1994-01-01 00:00:00

abstract：:The pH-related change in morphology of vinblastine (VLB)-induced paracrystals formed in Chinese hamster ovary (CHO) cells was examined immunohistochemically in order to determine both the mechanism of tubulin crystallization and the influence of acidic pHs on cytoskeletal microtubules. Lowering the extracellular pH (p...

journal_title：Biology of the cell

authors： Takanari H,Morita J,Yamanaka H,Yada K,Takahashi A,Izutsu K

更新日期：1994-01-01 00:00:00

abstract：:It is now well established that malignant transformation of eucaryotic cells is concomitant with typical alterations of glycosylation and the expression pattern of endogenous lectins. In parallel, oncogene transfection studies revealed a correlation between the expression of some of these genes, the transformed state ...

journal_title：Biology of the cell

authors： Hébert E,Monsigny M

更新日期：1993-01-01 00:00:00

abstract：:Flow cytometry has important advantages over conventional techniques. It is rapid, highly sensitive and allows multi-parametric analysis and cell sorting. Potential exists for the measurement of many cell functions by flow cytometry. The technique can be used to determine cell viability, intracellular calcium and pH, ...

journal_title：Biology of the cell

authors： Maftah A,Huet O,Gallet PF,Ratinaud MH

更新日期：1993-01-01 00:00:00

abstract：:Aspergillus fumigatus, the causative agent of human aspergillosis, binds to and degrades basement membrane laminin. Using immunoelectron microscopy, laminin binding appeared to be associated with the cell wall expansions of resting conidia, and progressively extended to the outer electron dense layer of the conidial w...

journal_title：Biology of the cell

authors： Tronchin G,Bouchara JP,Larcher G,Lissitzky JC,Chabasse D

更新日期：1993-01-01 00:00:00

abstract：:SaOS-2 cell line presents osteoblastic characteristics which can be modulated by specific agonists involving also phosphoinositide breakdown. In order to determine whether SaOS-2 cells display a phosphoinositide signalling system not only at the cytosol-cell membrane level but also, as recently reported for other cell...

journal_title：Biology of the cell

authors： Maraldi NM,Zini N,Santi S,Bavelloni A,Valmori A,Marmiroli S,Ognibene A

更新日期：1993-01-01 00:00:00

abstract：:Flow cytometry (FCM) analysis of cellular DNA content has become an increasingly important clinical research tool for the measurement and identification of abnormal cell populations. Examination of cellular DNA content can provide information for use in cell cycle analysis in cancer studies. Most researchers would agr...

journal_title：Biology of the cell

authors： Spyratos F

更新日期：1993-01-01 00:00:00

abstract：:The influence of triiodothyronine (T3) on avian myoblast proliferation and differentiation was studied in secondary cultures using plating densities of 2,500 and 7,000 cells/cm2. Culture media were depleted of T3 (control myoblasts) and increasing amounts were then added to concentrations of 0.6, 3 and 15 nM T3 (treat...

journal_title：Biology of the cell

authors： Marchal S,Cassar-Malek I,Pons F,Wrutniak C,Cabello G

更新日期：1993-01-01 00:00:00

abstract：:Bistramide A, a new toxin isolated from a New Caledonian Urochordata, shows an antiproliferative effect on a non-small-cell lung carcinoma line in vitro and G1-blockade. In this work, the growth arrest induced by bistramide A was shown to be irreversible as assessed by growth kinetics of pretreated cells. Furthermore,...

journal_title：Biology of the cell

authors： Riou D,Roussakis C,Robillard N,Biard JF,Verbist JF

更新日期：1993-01-01 00:00:00

abstract：:The dodecasatellite is a 11/12 bp tandemly repeated sequence which is overrepresented, with regard to its genomic representation, in extrachromosomal circular DNAs from D melanogaster embryos. Here we show that a bacterial plasmid carrying a cluster of dodecasatellite is not able to replicate efficiently in cultured c...

journal_title：Biology of the cell

authors： Renault S,Degroote F,Picard G

更新日期：1993-01-01 00:00:00

abstract：:Isolated cells from the proximal tubule have previously been used to study specific renal physiological processes. Here we analysed the structural changes induced by mechanical isolation on rat proximal tubule cells. As major modifications we detected: 1) a redistribution of brush border microvilli, which spread out o...

journal_title：Biology of the cell

authors： Rebelo L,Carmo-Fonseca M,Moura TF

更新日期：1992-01-01 00:00:00

abstract：:When mouse peritoneal macrophages were made to adhere firmly on glass surface and then removed by sequential treatment with hypotonic triethanolamine and Nonidet P-40, a set of proteins were found to be left behind at the sites of adherent cells. Such glass-adherent proteins were detected as round or ellipsoidal patch...

journal_title：Biology of the cell

authors： Tomita M,Ishikawa H

更新日期：1992-01-01 00:00:00

abstract：:The activities of three mycotoxins isolated from the hyphomycete Metarhizium anisopliae: destruxin A, B, and E (DA, DB and DE) are described and compared in vitro on leukemic cells. Their antitumor effect was investigated by flow cytometry on growth, cell viability and cell cycle perturbation 48 h after destruxin expo...

journal_title：Biology of the cell

authors： Odier F,Vey A,Bureau JP

更新日期：1992-01-01 00:00:00

abstract：:cdc25 proteins are universally involved in the control of cell division. Using an original method of sequence analysis, cdc25 proteins from different sources were compared to protein phosphatases. Protein phosphatases could clearly be characterized as two distinct protein families, the phospho-seryl/threonyl phosphata...

journal_title：Biology of the cell

authors： Bellé R,Ollivier E,Guerrucci MA

更新日期：1992-01-01 00:00:00

abstract：:The entry into, and exit from, mitosis are controlled by a universal M-phase promoting factor (MPF) composed of at least p34cdc2 and a cyclin. Embryonic systems are convenient for studying the association and dissociation of the active MPF complex because oocytes and eggs are naturally arrested at a specific point of ...

journal_title：Biology of the cell

authors： Charbonneau M,Grandin N

更新日期：1992-01-01 00:00:00

abstract：:Ciliary receptor cells, typified by cilia or modified cilia, are very common in the animal kingdom. In addition to the cytoskeleton of their ciliary processes these receptors possess other specific prominent cytoskeletal elements. Two representative systems are presented: i) scolopidia, mechanosensitive sensilla of va...

journal_title：Biology of the cell

authors： Wolfrum U

更新日期：1992-01-01 00:00:00

abstract：:Immunofluorescent techniques have been used in the analysis of DNA-RNA hybrids occurrence and its relationship to transcriptional events on polytene chromosomes of Drosophila subobscura. We have studied the distribution of these hybrids on uninduced/induced chromosomes. Two different indirect immunofluorescence method...

journal_title：Biology of the cell

authors： Arbona M,Cuenca JB,de Frutos R

更新日期：1992-01-01 00:00:00

abstract：:Membrane fluidity of erythrocytes obtained from 15 children with trisomy 21 and 20 healthy controls were studied by measuring steady-state fluorescence anisotropy and fluorescence lifetime of 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) incorporated in hemog...

journal_title：Biology of the cell

authors： Kantar A,Giorgi PL,Curatola G,Fiorini R

更新日期：1992-01-01 00:00:00

abstract：:Dynamic secondary ion mass spectrometry (SIMS) has been utilised to study the post-mortem distribution of aluminium in air-dried frozen sections from unfixed, unstained human brain in order to minimise contamination of the tissue and avoid redistribution and extraction of endogenous tissue aluminium. Substrates, sputt...

journal_title：Biology of the cell

authors： Candy JM,Oakley AE,Mountfort SA,Taylor GA,Morris CM,Bishop HE,Edwardson JA

更新日期：1992-01-01 00:00:00

abstract：:We took advantage of one of the main possibilities of ion microscopy, ie isotopic analysis, to study the cellular distribution of molecules labelled either with carbon 14 or with stable isotopes of low natural abundance such as nitrogen 15 and deuterium. The surface of the sample is bombarded with an ion beam (O2+, Cs...

journal_title：Biology of the cell

authors： Hindie E,Coulomb B,Beaupain R,Galle P

更新日期：1992-01-01 00:00:00

abstract：:The diversity of microtubular networks was analyzed in quail oviduct and in Paramecium cells using conventional and confocal immunofluorescence as well as pre- and post-embedding EM immunocytochemistry with a variety of anti-tubulin antibodies. The 6-11B-1 monoclonal antibody, specific for the post-translational acety...

journal_title：Biology of the cell

authors： Adoutte A,Delgado P,Fleury A,Levilliers N,Lainé MC,Marty MC,Boisvieux-Ulrich E,Sandoz D

更新日期：1991-01-01 00:00:00

abstract：:This review is concerned with the formation during ciliogenesis of centrioles and basal bodies, primarily in epithelial multi-ciliated cells from the developing vertebrate respiratory and reproductive tracts. During ciliated cell differentiation, in these as well as in other cell types, cilium formation is preceded by...

journal_title：Biology of the cell

authors： Dirksen ER

更新日期：1991-01-01 00:00:00

abstract：:Bovine tracheal gland (BTG) cells in culture show an epithelial-fibroblastoid transition after several passages. To investigate these BTG cell phenotype changes, we studied the effects of both the culture medium and passage number on the expression of epithelial cytoskeletal proteins and glandular serous cell markers....

journal_title：Biology of the cell

authors： Benali R,Dupuit F,Chevillard M,Jacquot J,Haye B,Puchelle E

更新日期：1991-01-01 00:00:00

abstract：:The plasma membrane components of the parasitic protozoan Entamoeba histolytica, the causative agent of human invasive amebiasis, have been biochemically and immunologically characterized during the last decade. In addition, genes coding for certain surface proteins have been cloned. In spite of these advances, a unif...

journal_title：Biology of the cell

authors： Espinosa-Cantellano M,Martínez-Palomo A

更新日期：1991-01-01 00:00:00

abstract：:The distribution of viral RNA molecules in HeLa cells infected with adenovirus type 5 (Ad5) was determined by in situ hybridization at the ultrastructural level at an intermediate stage of nuclear transformation, when viral DNA synthetic activities were maximal but progeny viruses were still sparse. Transcription site...

journal_title：Biology of the cell

authors： Puvion-Dutilleul F,Puvion E

更新日期：1991-01-01 00:00:00

abstract：:Total mRNA extracted from the brain of a marine worm, Nereis diversicolor, were in vitro translated using 2 cell-free systems: rabbit reticulocyte lysate and wheat germ extract. Among numerous products newly translated in both systems, only one class of 70 kDa polypeptides immunoprecipitated when we used a mixture of ...

journal_title：Biology of the cell

authors： Guissi-Kadri S,Dhainaut-Courtois N,Porchet M,Curgy JJ

更新日期：1991-01-01 00:00:00

abstract：:A digitally-enhanced videomicroscopy study of rabbit gastric parietal cells in primary culture was performed using alternate observations with differential interference contrast and fluorescence optics of cells mounted and perfused on a temperature-controlled microscope stage. The effect of histamine, a physiological ...

journal_title：Biology of the cell

authors： Mangeat P,Gusdinar T,Sahuquet A,Hanzel DK,Forte JG,Magous R

更新日期：1990-01-01 00:00:00